![]() The value is defined by the substrate concentration at half-maximum velocity V max /2. ![]() With increasing substrate concentrations, the enzyme reactivity will asymptotically approach its maximum velocity. The Michaelis-Menten expression is commonly used to describe the rate (v) of the enzyme reaction. This biproduct can be quantified and observed by monitoring its maximum absorption wavelength. When an enzyme (E) binds with a substrate (S), an intermediate or enzyme/substrate complex (ES) is produced, which can further react and yield a by-product (P), shown in Scheme 1. The rates of these reactions can be accurately measured using a UV-Visible spectrophotometer. Introduction V-650 UV-Visible SpectrophotometerĮnzyme activity is frequently investigated in the medicinal, biochemistry, and food science research fields to elucidate the rate of which reaction occurs and the affinity of the enzyme-substrate interactions. Circular Dichroism Tips & Tricks for Biological Samples. ![]() ![]() Circularly Polarized Luminescence (CPL). ![]()
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